The shell of the core bead does not have mono-sized pores. In essence, you can use these core beads to scavenge, or cut away, small impurities from large and sensitive target entities, like virus capsids, enveloped viruses, or exosomes. This is the beauty of core bead technology: it allows processing of large sample volumes using a relatively small chromatography column. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations and limited diffusion of impurities out from the bead 1.
The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various impurities small enough to diffuse through the shell and enter the core. The inert shell combined with ligand‑activated core enables high sample load in combination with group separation of molecules. Capto™ Core bead technology includes a chromatography resin with ligand‑activated core and an outer inactive shell. By combining size separation and binding chromatography, Capto™ Core resins can be a good alternative to SEC resins for purification of exosomes or other large entities.įig 1. This structure enables dual functionality, in which smaller impurities that can diffuse through the shell are captured, while larger target entities are collected in the flowthrough 1. The core bead technology includes a chromatography resin with ligand‑activated core and an outer inactive shell (Fig. Capto™ Core beads were developed to address this bottleneck and intensify downstream processing of large entities. But this SEC step is often seen as a productivity bottleneck - low flow rates and limited sample loads can slow virus and exosome purification. Size-exclusion chromatography (SEC) is often used in the downstream processing of large and sensitive entities, such as viruses or extracellular vesicles (EVs or exosomes).
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